• 1920x830 Light microscope HS08597

    Analytical Laboratories

     

    The Experimental Medicine, Metabolic and Nutritional Physiology Research Group houses multiple state-of-the-art analytical research laboratories in the Medical School and embedded within the Division of Physiology, Pharmacology and Neuroscience of the School of Life Sciences.

    Our longstanding 'world leading' and 'internationally excellent' research pedigree, combined with a team of academic researchers and highly skilled technical research specialists enables us to deliver advanced in-house sample analysis. 

     

    Analytical Laboratories

    Location: D Floor and E Floor, Medical School, Queen's Medical Centre

    We have multiple state-of-the-art analytical laboratories to allow for in-house sample analysis using a wide range of specialised laboratory techniques.

     
    Some of our techniques:

    Our analytical laboratories are equipped to provide capability for:

    • General protein, DNA and RNA quantification using in-house extraction assays and Spectroscopy (Visible and UV). 

    • Specific protein quantification (detecting changes in the levels of specific protein molecules among a mixture of proteins) using Western blotting. 

    • Gene expression analysis (detecting changes in the level of expression of specific genes) using Real-time PCR. 

    • Live cell imaging (studying living cells over some time) and Fixed Cell imaging (studying lipid accumulation in muscle cells) using Fluorescence Microscopy. 

    • Substrate and metabolite analysis using a variety of techniques including spectrophotometry, fluorimetry, ELISAs, magnetic bead flow cytometry, and in-house radiochemical assays. 

    • Mitochondrial respiration measurements using respirometry and luminescence. 

     

    Experimental Medicine, Metabolic and Nutritional Physiology Research Group

    David Greenfield Human Physiology Unit
    School of Life Sciences
    Medical School
    University of Nottingham
    Nottingham, NG7 2UH